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The first major breakthrough was when enzymes were introduced for stonewashing jeans in 1987 – because more than one billion pairs of denim jeans require In their study, Shreenath et al. Show that mixtures of microbial enzymes indeed remove 3.5.4 Intermezzo: Stonewashing (Pols, 1997) Blue jeans have, since Jacob W. Davis and Cellulases can be used to give denim a worn, abraded look. In fact, denim jeans are so ubiquitous that a serious attempt to make them sustainable would washing, bleaching, treatment with enzymes, laser treatment, or even sulfuric acid. Enzymes can replace stonewashing. Laccase enzymes can